The role of NbTMP1, a surface protein of sporoplasm, in Nosema bombycis infection

Abstract Background Nosema bombycis is a unicellular eukaryotic pathogen of the silkworm, Bombyx mori , and an economic occupational hazard in silkworm industry. Because its long incubation period horizontal vertical transmission, it subject to quarantine measures sericulture production. The microsporidian life-cycle includes dormant extracellular phase intracellular proliferation phase, with being most active period. This latter lacks spore wall protection may be susceptible stage for control. Methods In order find suitable target selective breeding N. -resistant strains, we screen highly expressed membrane proteins from transcriptome data . subcellular localization candidate protein was verified by Indirect immunofluorescence analysis (IFA) immunoelectron microscopy (IEM), role RNAi. Results (NBO_76g0014) identified as transmembrane named NbTMP1. It homologous hypothetical NGRA_1734 granulosis NbTMP1 has region 23 amino acids at N-terminus. results suggest that secreted on plasma spores develop. Western blot qRT-PCR showed all developmental stages infected cells midgut. Downregulation expression resulted significant inhibition proliferation. Conclusions We confirmed bombycis. Reduction transcription level significantly inhibited proliferation, this strains. Graphical